STUDIES OF MEMBRANE FORMATION IN TETRAHYMENA PYRIFORMIS II . Isolation and Lipid Analysis of Cell Fractions
نویسنده
چکیده
A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant . The method prevents the destructive action of lipolytic enzymes commonly associated with this organism . Analysis of the membrane lipids of these fractions reveals significant differences in lipid composition . Most noteworthy are the high concentrations of phosphonolipid and tetrahymanol in the surface membranes.
منابع مشابه
Studies of Membrane Formation in Tetrahymena Pyriformis
A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals ...
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When 114C-palmitic acid is used to pulse label logarithmic cultures of Tetrahymena pyriformis, radioactivity appears in lipids of the various membrane types at vastly differing rates . The microsomes and postmicrosomal supernatant attain a high specific radioactivity within 1 min, while the membranes enveloping the cilia require several hours to reach the microsomal level. A similar pattern is ...
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تاریخ انتشار 2003